› 5syd

X-ray diffraction
2.4Å resolution

Circularly permutated azurin (cpAz) based on P. aeruginosa azurin sequence

Released:
DOI: 10.2210/pdb5syd/pdb
PDB 5syd coloured by chain and viewed from the front.
PDB 5syd coloured by chain and viewed from the side.
PDB 5syd coloured by chain and viewed from the top.
Source organisms:
Entry authors: Petrik I, Yang Y, Howard R, Yi L

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.08 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3EWN9 (Residues: 43-109; Coverage: 52%)
  • Canonical: P00282 (Residues: 130-148; Coverage: 15%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 115.436Å b: 115.436Å c: 83.145Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.224 0.251
Expression system: Escherichia coli BL21(DE3)

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