PDBe 5sy7

X-ray diffraction
4.2Å resolution

Crystal Structure of the Heterodimeric NPAS3-ARNT Complex with HRE DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Aryl hydrocarbon receptor nuclear translocator Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53762 (Residues: 81-464; Coverage: 49%)
Gene name: Arnt
Sequence domains:
Neuronal PAS domain-containing protein 3 Chain: B
Molecule details ›
Chain: B
Length: 410 amino acids
Theoretical weight: 46.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QZQ0 (Residues: 56-455; Coverage: 43%)
Gene names: Mop6, Npas3
Sequence domains:
DNA (5'-D(*GP*GP*CP*TP*GP*CP*GP*TP*AP*CP*GP*TP*GP*CP*GP*GP*GP*TP*CP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
DNA (5'-D(*CP*AP*CP*GP*AP*CP*CP*CP*GP*CP*AP*CP*GP*TP*AP*CP*GP*CP*AP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.36 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 64.831Å b: 64.831Å c: 249.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.272 0.361
Expression system: Escherichia coli BL21(DE3)