› 5rnt

X-ray diffraction
3.2Å resolution

X-RAY ANALYSIS OF CUBIC CRYSTALS OF THE COMPLEX FORMED BETWEEN RIBONUCLEASE T1 AND GUANOSINE-3',5'-BISPHOSPHATE

Released:
DOI: 10.2210/pdb5rnt/pdb
PDB entry 5rnt coloured by chain, front view.
PDB entry 5rnt coloured by chain, side view.
PDB entry 5rnt coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
X-ray analysis of cubic crystals of the complex formed between ribonuclease T1 and guanosine-3',5'-bisphosphate.
Lenz A, Heinemann U, Maslowska M, Saenger W
Acta Crystallogr B 47 ( Pt 4) 521-7 (1991)
PMID: 1930833

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Not provided
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand PGP 1 x PGP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I23
Unit cell:
a: 86.47Å b: 86.47Å c: 86.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 not available not available
Expression system: Not provided

Quick links

Citations

9 citation in other articles

TESS: a geometric hashing algorithm for deriving 3D coordinate templates for searching structural databases. Application to enzyme active sites.
Wallace et al. (1997)
8 more

2 mentions without citation

Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER.
Smart et al. (2012)
1 more