5qox

X-ray diffraction
1.95Å resolution

PanDDA analysis group deposition -- Crystal Structure of DCP2 (NUDT20) in complex with Z2212124043

Released:
Source organism: Homo sapiens
Entry authors: Nelson ER, Velupillai S, Talon R, Collins PM, Krojer T, Wang D, Brandao-Neto J, Douangamath A, Burgess-Brown N, Arrowsmith CH, Bountra C, Huber K, von Delft F

Function and Biology Details

Reaction catalysed:
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-diphosphate + 5'-phospho-[mRNA]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
m7GpppN-mRNA hydrolase Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IU60 (Residues: 95-260; Coverage: 40%)
Gene names: DCP2, NUDT20
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 48.201Å b: 60.331Å c: 65.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.241
Expression system: Escherichia coli