5qib

X-ray diffraction
1.48Å resolution

PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with FMOPL000388a

Released:
Source organism: Homo sapiens
Entry authors: MacKinnon S, Bezerra GA, Krojer T, Bradley AR, Talon R, Brandao-Neto J, Douangamath A, von Delft F, Arrowsmith CH, Edwards A, Bountra C, Oppermann U, Brennan PE, Yue WW

Function and Biology Details

Reactions catalysed:
Glyoxylate + H(2)O + O(2) = oxalate + H(2)O(2)
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-Hydroxyacid oxidase 1 Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 40.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UJM8 (Residues: 1-368; Coverage: 100%)
Gene names: GOX1, HAO1
Sequence domains: FMN-dependent dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FNR 1 x FNR
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I4
Unit cell:
a: 97.996Å b: 97.996Å c: 81.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.209
Expression system: Escherichia coli