5pan

X-ray diffraction
1.62Å resolution

Crystal Structure of Factor VIIa in complex with 5-hydroxy-N-(3-oxo-1,2-dihydroisoindol-5-yl)-1-[3-[(phenylcarbamoylamino)methyl]phenyl]pyrazole-4-carboxamide

Released:
Source organism: Homo sapiens
Entry authors: Stihle M, Mayweg A, Roever S, Rudolph MG

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Factor VII light chain Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 6.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08709 (Residues: 149-212; Coverage: 14%)
Gene name: F7
Structure domains: Laminin
Factor VII heavy chain Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 95.28Å b: 95.28Å c: 116.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.179
Expression system: Escherichia coli