5oxj

X-ray diffraction
2Å resolution

Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a O6-MeG:BenziTP base pair

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119118 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 60.93 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 293-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
Structure domains:
DNA primer Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA template Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 109.347Å b: 109.347Å c: 90.636Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.257
Expression systems:
  • Escherichia coli
  • Not provided