› 5oxb

X-ray diffraction
1.38Å resolution

Structure of blue-light irradiated Cerulean

Released:
DOI: 10.2210/pdb5oxb/pdb
PDB 5oxb coloured by chain and viewed from the front.
PDB 5oxb coloured by chain and viewed from the side.
PDB 5oxb coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins.
Gotthard G, von Stetten D, Clavel D, Noirclerc-Savoye M, Royant A
Biochemistry 56 6418-6422 (2017)
PMID: 29148725

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.79 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand B2H 1 x B2H

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.06Å b: 62.66Å c: 69.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.128 0.157
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.
Di Costanzo et al. (2021)
3 other articles

1 mention without citation

Luminescence- and Fluorescence-Based Complementation Assays to Screen for GPCR Oligomerization: Current State of the Art.
Wouters et al. (2019)