5oxa

X-ray diffraction
1.16Å resolution

Structure of the S205A mutant of the Cyan Fluorescent Protein Cerulean at pH 7.0

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.78 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 50.96Å b: 63.32Å c: 69.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.116 0.139
Expression system: Escherichia coli BL21