X-ray diffraction
1.87Å resolution

Bacillus megaterium porphobilinogen deaminase D82N mutant

Source organism: Priestia megaterium

Function and Biology Details

Reaction catalysed:
4 porphobilinogen + H(2)O = hydroxymethylbilane + 4 NH(3)
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Porphobilinogen deaminase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 34.29 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
  • Canonical: Q8GCA8 (Residues: 1-310; Coverage: 100%)
Gene names: C3744_10255, hemC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 48.99Å b: 62.69Å c: 91.26Å
α: 90° β: 90° γ: 90°
R R work R free
0.212 0.21 0.253
Expression system: Escherichia coli