› 5osx

X-ray diffraction
1.92Å resolution

Translation initiation factor 4E in complex with m7G(5'S)ppp(5'S)G mRNA 5' cap analog

Released:
DOI: 10.2210/pdb5osx/pdb
PDB entry 5osx coloured by chain, front view.
PDB entry 5osx coloured by chain, side view.
PDB entry 5osx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
5'-Phosphorothiolate Dinucleotide Cap Analogues: Reagents for Messenger RNA Modification and Potent Small-Molecular Inhibitors of Decapping Enzymes.
Wojtczak BA, Sikorski PJ, Fac-Dabrowska K, Nowicka A, Warminski M, Kubacka D, Nowak E, Nowotny M, Kowalska J, Jemielity J
J Am Chem Soc 140 5987-5999 (2018)
PMID: 29676910

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 22.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63073 (Residues: 28-217; Coverage: 88%)
Gene name: Eif4e
Sequence domains: Eukaryotic initiation factor 4E

Ligands and Environments

2 bound ligands:
Ligand AKW 2 x AKW
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 37.843Å b: 37.855Å c: 145.987Å
α: 86.56° β: 84.56° γ: 77.05°
R-values:
R R work R free
0.226 0.224 0.269
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery.
Kowalski et al. (2019)
9 more