› 5osq

X-ray diffraction
2.05Å resolution

ZP-N domain of mammalian sperm receptor ZP3 (crystal form II, processed in P21221)

Released:
DOI: 10.2210/pdb5osq/pdb
PDB 5osq coloured by chain and viewed from the front.
PDB 5osq coloured by chain and viewed from the side.
PDB 5osq coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats.
Monné M, Han L, Schwend T, Burendahl S, Jovine L
Nature 456 653-7 (2008)
PMID: 19052627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Processed zona pellucida sperm-binding protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 52.95 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
  • Canonical: P10761 (Residues: 42-143; Coverage: 25%)
Gene names: JW3994, Zp-3, Zp3, Zpc, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CA 6 x CA
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Unit cell:
a: 91.34Å b: 92.16Å c: 140.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Mammalian egg coat modifications and the block to polyspermy.
Fahrenkamp et al. (2020)
23 more

1 mention without citation

Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.
Nishimura et al. (2019)