› 5oqt

X-ray diffraction
2.86Å resolution

Crystal structure of a bacterial cationic amino acid transporter (CAT) homologue

Released:
DOI: 10.2210/pdb5oqt/pdb
PDB entry 5oqt coloured by chain, front view.
PDB entry 5oqt coloured by chain, side view.
PDB entry 5oqt coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for amino acid transport by the CAT family of SLC7 transporters.
Jungnickel KEJ, Parker JL, Newstead S
Nat Commun 9 550 (2018)
PMID: 29416041

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107540 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Amino acid transporter Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 50.81 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5L1G5 (Residues: 1-471; Coverage: 100%)
Gene name: GK0930
Sequence domains: Amino acid permease
Small protein MgtS Chain: C
Molecule details ›
Chain: C
Length: 31 amino acids
Theoretical weight: 3.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5A616 (Residues: 1-31; Coverage: 100%)
Gene names: JW1527.1, b4599, mgtS, yneM

Ligands and Environments

5 bound ligands:
Ligand OLC 13 x OLC
Ligand ACT 1 x ACT
Ligand CL 1 x CL
Ligand ALA 1 x ALA
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 76.923Å b: 83.148Å c: 119.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Arginine-dependent immune responses.
Martí I Líndez et al. (2021)
13 more

12 mentions without citation

Rush Hour of LATs towards Their Transport Cycle.
Nicolàs-Aragó et al. (2021)
11 more