5opx

X-ray diffraction
3.64Å resolution

Crystal structure of the GroEL mutant A109C in complex with GroES and ADP BeF2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 548 amino acids
Theoretical weight: 57.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F5 (Residues: 1-548; Coverage: 100%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:
Co-chaperonin GroES Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 97 amino acids
Theoretical weight: 10.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6F9 (Residues: 1-97; Coverage: 100%)
Gene names: JW4102, b4142, groES, groS, mopB
Sequence domains: Chaperonin 10 Kd subunit
Structure domains: GroES chaperonin

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 217.567Å b: 230.504Å c: 235.391Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.256
Expression system: Escherichia coli BL21(DE3)