5oob

X-ray diffraction
2.79Å resolution

COMPLEX OF HUMAN NUCLEAR CAP-BINDING COMPLEX WITH M7GTP AND NELF-E C-TERMINAL PEPTIDE

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear cap-binding protein subunit 1 Chains: A, C, F, I
Molecule details ›
Chains: A, C, F, I
Length: 772 amino acids
Theoretical weight: 89.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q09161 (Residues: 20-790; Coverage: 98%)
Gene names: CBP80, NCBP, NCBP1
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Nuclear cap-binding protein subunit 2 Chains: B, D, G, J
Molecule details ›
Chains: B, D, G, J
Length: 158 amino acids
Theoretical weight: 18.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52298 (Residues: 1-156; Coverage: 100%)
Gene names: CBP20, NCBP2, PIG55
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
Negative elongation factor E Chains: E, K, Z
Molecule details ›
Chains: E, K, Z
Length: 21 amino acids
Theoretical weight: 2.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: L9KL62 (Residues: 412-432; Coverage: 5%)
Gene name: TREES_T100017785

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21
Unit cell:
a: 113.8Å b: 147.23Å c: 153.88Å
α: 90° β: 91.48° γ: 90°
R-values:
R R work R free
0.203 0.202 0.229
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided