› 5onm

X-ray diffraction
1.52Å resolution

Crystal Structure of Ectoine Synthase from P. lautus

Released:
DOI: 10.2210/pdb5onm/pdb
PDB entry 5onm coloured by chain, front view.
PDB entry 5onm coloured by chain, side view.
PDB entry 5onm coloured by chain, top view.
Source organism: Paenibacillus lautus
Primary publication:
Illuminating the catalytic core of ectoine synthase through structural and biochemical analysis.
Czech L, Höppner A, Kobus S, Seubert A, Riclea R, Dickschat JS, Heider J, Smits SHJ, Bremer E
Sci Rep 9 364 (2019)
PMID: 30674920

Function and Biology Details

Reaction catalysed: 
N(4)-acetyl-L-2,4-diaminobutanoate = L-ectoine + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-ectoine synthase Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.59 KDa
Source organism: Paenibacillus lautus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1R1AV52 (Residues: 1-130; Coverage: 100%)
Gene names: BK123_26285, ectC
Sequence domains: Ectoine synthase

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 71.13Å b: 71.13Å c: 68.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.203
Expression system: Escherichia coli

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Citations

2 review citations

Bioactivity profiling of the extremolyte ectoine as a promising protectant and its heterologous production.
Ma et al. (2022)
1 more

2 mentions without citation

Illuminating the catalytic core of ectoine synthase through structural and biochemical analysis.
Czech et al. (2019)
1 more