X-ray diffraction
2.6Å resolution

Mutant T252A of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucyl-adenylate analogue in the aminoacylation conformation


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Leucine--tRNA ligase Chains: A, D
Molecule details ›
Chains: A, D
Length: 880 amino acids
Theoretical weight: 99.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
  • Canonical: P07813 (Residues: 1-860; Coverage: 100%)
Gene names: JW0637, b0642, leuS
Sequence domains:
L-leucyl-tRNA Chains: B, E
Molecule details ›
Chains: B, E
Length: 87 nucleotides
Theoretical weight: 28.05 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 158.25Å b: 68.91Å c: 228.33Å
α: 90° β: 104.93° γ: 90°
R R work R free
0.221 0.219 0.264
Expression systems:
  • Escherichia coli BL21
  • Not provided