5omq

X-ray diffraction
2.2Å resolution

Ternary complex of 9N DNA polymerase in the replicative state with three metal ions in the active site

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 775 amino acids
Theoretical weight: 89.83 KDa
Source organism: Thermococcus sp. 9oN-7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56366 (Residues: 1-775; Coverage: 100%)
Gene names: pol, polA
Sequence domains:
Structure domains:
DNA Template Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA Primer Chain: P
Molecule details ›
Chain: P
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 66.793Å b: 93.678Å c: 158.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.248
Expression systems:
  • Escherichia coli
  • Not provided