› 5olx

X-ray diffraction
1.38Å resolution

5-fluorotryptophan labeled beta-phosphoglucomutase in a closed conformation, orthorhomic crystal form

Released:
DOI: 10.2210/pdb5olx/pdb
PDB entry 5olx coloured by chain, front view.
PDB entry 5olx coloured by chain, side view.
PDB entry 5olx coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Primary publication:
Observing enzyme ternary transition state analogue complexes by 19F NMR spectroscopy.
Ampaw A, Carroll M, von Velsen J, Bhattasali D, Cohen A, Bowler MW, Jakeman DL
Chem Sci 8 8427-8434 (2017)
PMID: 29619190

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.28 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P71447 (Residues: 1-221; Coverage: 100%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand BG6 1 x BG6
Ligand MGF 4 x MGF
1 modified residue:
Ligand FTR 2 x FTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 37.53Å b: 54.164Å c: 104.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.154 0.209
Expression system: Escherichia coli BL21

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Citations

1 mention without citation

Observing enzyme ternary transition state analogue complexes by 19F NMR spectroscopy.
Ampaw et al. (2017)