X-ray diffraction
1.25Å resolution

Non-conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with cellobiose-6-phosphate


Function and Biology Details

Reaction catalysed:
A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Putative 6-phospho-beta-galactobiosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 485 amino acids
Theoretical weight: 56.21 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
  • Canonical: W8QF82 (Residues: 2-478; Coverage: 100%)
Gene name: gan1D
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BG6, BGC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 101.599Å b: 97.48Å c: 105.269Å
α: 90° β: 97.66° γ: 90°
R R work R free
0.139 0.139 0.158
Expression system: Escherichia coli