5okf

X-ray diffraction
3.2Å resolution

CH1 chimera of human 14-3-3 sigma with the HSPB6 phosphopeptide in a conformation with self-bound phosphopeptides

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14-3-3 protein sigma; Heat shock protein beta-6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 27.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14558 (Residues: 12-19; Coverage: 5%)
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, HSPB6, SFN
Sequence domains: 14-3-3 protein

Ligands and Environments

1 bound ligand:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 77.399Å b: 97.76Å c: 158.831Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.247 0.279
Expression system: Escherichia coli