› 5ojy

X-ray diffraction
1.85Å resolution

Co-complex structure of regulator protein 2 (PamR2) with pamamycin 607 from Streptomyces alboniger

Released:
DOI: 10.2210/pdb5ojy/pdb
PDB 5ojy coloured by chain and viewed from the front.
PDB 5ojy coloured by chain and viewed from the side.
PDB 5ojy coloured by chain and viewed from the top.
Source organism: Streptomyces alboniger
Primary publication:
Design, development and application of whole-cell based antibiotic-specific biosensor.
Rebets Y, Schmelz S, Gromyko O, Tistechok S, Petzke L, Scrima A, Luzhetskyy A
Metab Eng 47 263-270 (2018)
PMID: 29609044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.28 KDa
Source organism: Streptomyces alboniger
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0D3RJT7 (Residues: 2-245; Coverage: 100%)
Gene names: CP975_33745, SALB_00464, pamR2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand CIT 1 x CIT
Ligand 9XB 1 x 9XB
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6222
Unit cell:
a: 107.85Å b: 107.85Å c: 119.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.196
Expression system: Escherichia coli

Quick links

Citations

5 review citations

An Update on Molecular Tools for Genetic Engineering of Actinomycetes-The Source of Important Antibiotics and Other Valuable Compounds.
Mitousis et al. (2020)
4 more