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Crystal structure of USP30 in covalent complex with ubiquitin propargylamide (low resolution)

X-ray diffraction
3.6Å resolution
PDB entry 5ohn coloured by chain, front view.
PDB entry 5ohn coloured by chain, side view.
PDB entry 5ohn coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ubiquitin carboxyl-terminal hydrolase 30 and Ubiquitin
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Multimeric state: hetero dimer
Accessible surface area: 16294.18 Å2
Buried surface area: 3162.37 Å2
Dissociation area: 1,581.19 Å2
Dissociation energy (ΔGdiss): 7.51 kcal/mol
Dissociation entropy (TΔSdiss): 11.36 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143281
Assembly 2
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Assembly Name: Ubiquitin carboxyl-terminal hydrolase 30 and Ubiquitin
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Multimeric state: hetero dimer
Accessible surface area: 16178.22 Å2
Buried surface area: 3155.02 Å2
Dissociation area: 1,577.51 Å2
Dissociation energy (ΔGdiss): 7.64 kcal/mol
Dissociation entropy (TΔSdiss): 11.36 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143281

Macromolecules

Ubiquitin carboxyl-terminal hydrolase 30
Chains: A, C
Length: 370 amino acids
Theoretical weight: 42.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70CQ3 (Residues: 64-357, 432-502; Coverage: 71%)
Gene name: USP30
Pfam: Ubiquitin carboxyl-terminal hydrolase
InterPro:
Ubiquitin carboxyl-terminal hydrolase 30 in PDB entry 5ohn, assembly 1, front view.
Ubiquitin carboxyl-terminal hydrolase 30 in PDB entry 5ohn, assembly 1, side view.
Ubiquitin carboxyl-terminal hydrolase 30 in PDB entry 5ohn, assembly 1, top view.
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Ubiquitin
Chains: B, D
Length: 76 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Pfam: Ubiquitin family
InterPro:
Ubiquitin in PDB entry 5ohn, assembly 1, front view.
Ubiquitin in PDB entry 5ohn, assembly 1, side view.
Ubiquitin in PDB entry 5ohn, assembly 1, top view.
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