5oeo

Solution NMR

Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Transient receptor potential cation channel subfamily V member 5 Chain: C
Molecule details ›
Chain: C
Length: 73 amino acids
Theoretical weight: 7.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NQA5 (Residues: 655-725; Coverage: 10%)
Gene names: ECAC1, TRPV5

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 75%
Refinement method: simulated annealing
Chemical shifts: BMR34161  
Expression system: Escherichia coli BL21