5oec

X-ray diffraction
2.3Å resolution

Human Rab32 (18-201):GDP in complex with Salmonella GtgE (21-214) C45A mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-171804 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GtgE Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.6 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6EAT3 (Residues: 21-214; Coverage: 85%)
Gene names: A7D45_11990, AL463_22290, AZF90_21570, CQW68_16290, D3Q81_09245, DT651_08660, EKD96_19760, F1527_14270, F2D26_09345, FJR52_13185, GCH85_24895, gtgE
Ras-related protein Rab-32 Chain: B
Molecule details ›
Chain: B
Length: 183 amino acids
Theoretical weight: 20.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13637 (Residues: 20-201; Coverage: 81%)
Gene name: RAB32
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 47.822Å b: 66.993Å c: 110.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.245
Expression system: Escherichia coli