5ocu

Electron Microscopy
5.2Å resolution

Molecular basis of human kinesin-8 function and inhibition

Released:
Primary publication:
Structural basis of human kinesin-8 function and inhibition.
OpenAccess logo Proc. Natl. Acad. Sci. U.S.A. (2017)
PMID: 29078367
Related structures: EMD-3780

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-like protein KIF18A Chain: K
Molecule details ›
Chain: K
Length: 377 amino acids
Theoretical weight: 42.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NI77 (Residues: 1-374; Coverage: 42%)
Gene names: KIF18A, OK/SW-cl.108
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.2Å
Relevant EMDB volumes: EMD-3780
Expression system: Escherichia coli BL21(DE3)