› 5obz

X-ray diffraction
3.7Å resolution

low resolution structure of the p34ct/p44ct minimal complex

Released:
DOI: 10.2210/pdb5obz/pdb
PDB entry 5obz coloured by chain, front view.
PDB entry 5obz coloured by chain, side view.
PDB entry 5obz coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH.
Radu L, Schoenwetter E, Braun C, Marcoux J, Koelmel W, Schmitt DR, Kuper J, Cianférani S, Egly JM, Poterszman A, Kisker C
Nucleic Acids Res 45 10872-10883 (2017)
PMID: 28977422

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123879 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General transcription and DNA repair factor IIH subunit TFB4 Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 31.97 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0RXV8 (Residues: 1-277; Coverage: 65%)
Gene name: CTHT_0004460
Sequence domains: Transcription factor Tfb4
Structure domains: von Willebrand factor, type A domain
General transcription and DNA repair factor IIH Chain: B
Molecule details ›
Chain: B
Length: 167 amino acids
Theoretical weight: 17.85 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0RZE6 (Residues: 368-534; Coverage: 31%)
Gene name: CTHT_0002690
Sequence domains: TFIIH C1-like domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6322
Unit cell:
a: 138.212Å b: 138.212Å c: 95.235Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The essential and multifunctional TFIIH complex.
Rimel et al. (2018)
7 other articles

2 mentions without citation

The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH.
Radu et al. (2017)
1 more