› 5ob0

X-ray diffraction
1.17Å resolution

Crystal structure of the c-Src-SH3 domain Q128E mutant in complex with the high affinity peptide APP12

Released:
DOI: 10.2210/pdb5ob0/pdb
PDB entry 5ob0 coloured by chain, front view.
PDB entry 5ob0 coloured by chain, side view.
PDB entry 5ob0 coloured by chain, top view.
Source organisms:
Entry author: Camara-Artigas A

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132650 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.91 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00523 (Residues: 85-141; Coverage: 11%)
Gene name: SRC
Sequence domains: SH3 domain
Structure domains: SH3 Domains
APP12 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Unit cell:
a: 31.63Å b: 32.56Å c: 65.262Å
α: 90° β: 103.02° γ: 90°
R-values:
R R work R free
0.132 0.131 0.149
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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