Structure analysis

Structure of nucleosome-Chd1 complex

Electron Microscopy
4.8Å resolution
Assembly composition:
hetero undecamer (preferred)
Entry contents: 6 distinct polypeptide molecules
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero undecamer
Accessible surface area: 128511.28 Å2
Buried surface area: 60908.9 Å2
Dissociation area: 4,149.48 Å2
Dissociation energy (ΔGdiss): 59.2 kcal/mol
Dissociation entropy (TΔSdiss): 12.8 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-119088

Macromolecules

Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:

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Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Pfam: Centromere kinetochore component CENP-T histone fold
InterPro:

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Chains: C, G
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Pfam:
InterPro:

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Chain: D
Length: 123 amino acids
Theoretical weight: 13.66 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:

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Chain: H
Length: 122 amino acids
Theoretical weight: 13.53 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-125; Coverage: 96%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:

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Name: DNA (162-MER)
Representative chains: I
Length: 208 nucleotides
Theoretical weight: 64.14 KDa

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Name: DNA (162-MER)
Representative chains: J
Length: 208 nucleotides
Theoretical weight: 64.29 KDa

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