5o9g

Electron Microscopy
4.8Å resolution

Structure of nucleosome-Chd1 complex

Released:
Primary publication:
Nucleosome-Chd1 structure and implications for chromatin remodelling.
OpenAccess logo Nature 550 539-542 (2017)
PMID: 29019976
Related structures: EMD-3765

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Histone H2B 1.1 Chain: D
Molecule details ›
Chain: D
Length: 123 amino acids
Theoretical weight: 13.66 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Histone H2B 1.1 Chain: H
Molecule details ›
Chain: H
Length: 122 amino acids
Theoretical weight: 13.53 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-125; Coverage: 96%)
Chromo domain-containing protein 1 Chain: W
Molecule details ›
Chain: W
Length: 1468 amino acids
Theoretical weight: 168.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P32657 (Residues: 1-1468; Coverage: 100%)
Gene names: CHD1, SYGP-ORF4, YER164W
Sequence domains:
DNA (162-MER) Chain: I
Molecule details ›
Chain: I
Length: 208 nucleotides
Theoretical weight: 64.14 KDa
DNA (162-MER) Chain: J
Molecule details ›
Chain: J
Length: 208 nucleotides
Theoretical weight: 64.29 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-3765
Expression systems:
  • Escherichia coli
  • Trichoplusia ni