5o98

X-ray diffraction
1.55Å resolution

Binary complex of Catharanthus roseus Vitrosamine Synthase with NADP+

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 34.51 KDa
Source organism: Catharanthus roseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0C5DR25 (Residues: 2-299; Coverage: 100%)
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 58.83Å b: 61.02Å c: 162.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.19
Expression system: Escherichia coli BL21(DE3)