X-ray diffraction
3.2Å resolution

Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 and pregnanolone

Source organisms:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Gamma-aminobutyric acid receptor subunit alpha-5; Gamma-aminobutyric acid receptor subunit beta-3 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 367 amino acids
Theoretical weight: 41.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
  • Canonical: P31644 (Residues: 261-346, 424-462; Coverage: 29%)
  • Canonical: P28472 (Residues: 26-246; Coverage: 49%)
Gene names: GABRA5, GABRB3
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:
Nanobody Nb25 Chains: K, O
Molecule details ›
Chains: K, O
Length: 125 amino acids
Theoretical weight: 13.67 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Nanobody Nb25 Chains: L, M, N
Molecule details ›
Chains: L, M, N
Length: 124 amino acids
Theoretical weight: 13.59 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I23
Unit cell:
a: 290.102Å b: 290.102Å c: 290.102Å
α: 90° β: 90° γ: 90°
R R work R free
0.233 0.232 0.242
Expression systems:
  • Homo sapiens
  • Escherichia coli