› 5o6b

X-ray diffraction
2.03Å resolution

Structure of ScPif1 in complex with GGGTTTT and ADP-AlF4

Released:
DOI: 10.2210/pdb5o6b/pdb
PDB entry 5o6b coloured by chain, front view.
PDB entry 5o6b coloured by chain, side view.
PDB entry 5o6b coloured by chain, top view.
Source organisms:
Primary publication:
Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.
Lu KY, Chen WF, Rety S, Liu NN, Wu WQ, Dai YX, Li D, Ma HY, Dou SX, Xi XG
Nucleic Acids Res 46 1486-1500 (2018)
PMID: 29202194

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119082 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ATP-dependent DNA helicase PIF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 545 amino acids
Theoretical weight: 61.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P07271 (Residues: 237-780; Coverage: 63%)
Gene names: PIF1, TST1, YM9958.01C, YML061C
Sequence domains:
DNA (5'-D(*GP*GP*GP*TP*TP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 75.246Å b: 88.373Å c: 187.703Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.238
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Pif1 family DNA helicases: A helpmate to RNase H?
Pohl et al. (2019)
5 more

6 mentions without citation

The three-component helicase/primase complex of herpes simplex virus-1.
Bermek et al. (2021)
5 more