5o2a

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reactions catalysed:
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Bifunctional protein FolD Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 31.2 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:
Bifunctional protein FolD Chain: B
Molecule details ›
Chain: B
Length: 289 amino acids
Theoretical weight: 31.2 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:
Bifunctional protein FolD Chain: C
Molecule details ›
Chain: C
Length: 289 amino acids
Theoretical weight: 31.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:
Bifunctional protein FolD Chain: D
Molecule details ›
Chain: D
Length: 289 amino acids
Theoretical weight: 31.15 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 100.106Å b: 79.393Å c: 101.776Å
α: 90° β: 113.8° γ: 90°
R-values:
R R work R free
0.167 0.166 0.181
Expression system: Escherichia coli BL21(DE3)