5o22

X-ray diffraction
2.1Å resolution

E. coli FolD in complex with carolacton

Released:

Function and Biology Details

Reactions catalysed:
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Bifunctional protein FolD Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 31.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:
Bifunctional protein FolD Chain: B
Molecule details ›
Chain: B
Length: 289 amino acids
Theoretical weight: 31.22 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:
Bifunctional protein FolD Chain: C
Molecule details ›
Chain: C
Length: 289 amino acids
Theoretical weight: 31.16 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:
Bifunctional protein FolD Chain: D
Molecule details ›
Chain: D
Length: 289 amino acids
Theoretical weight: 31.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24186 (Residues: 2-288; Coverage: 100%)
Gene names: JW0518, ads, b0529, folD
Sequence domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 99.64Å b: 81.016Å c: 100.935Å
α: 90° β: 112.95° γ: 90°
R-values:
R R work R free
0.176 0.175 0.206
Expression system: Escherichia coli BL21(DE3)