› 5o1a

X-ray diffraction
1.44Å resolution

p53 cancer mutant Y220C in complex with compound MB240

Released:
DOI: 10.2210/pdb5o1a/pdb
PDB 5o1a coloured by chain and viewed from the front.
PDB 5o1a coloured by chain and viewed from the side.
PDB 5o1a coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Aminobenzothiazole derivatives stabilize the thermolabile p53 cancer mutant Y220C and show anticancer activity in p53-Y220C cell lines.
Baud MGJ, Bauer MR, Verduci L, Dingler FA, Patel KJ, Horil Roy D, Joerger AC, Fersht AR
Eur J Med Chem 152 101-114 (2018)
PMID: 29702446

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-312; Coverage: 56%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand 9H5 2 x 9H5
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 65.156Å b: 71.087Å c: 105.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.178
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Advanced Strategies for Therapeutic Targeting of Wild-Type and Mutant p53 in Cancer.
Zhang et al. (2022)
7 more

1 mention without citation

Aminobenzothiazole derivatives stabilize the thermolabile p53 cancer mutant Y220C and show anticancer activity in p53-Y220C cell lines.
Baud et al. (2018)