X-ray diffraction
1.73Å resolution

Complex of H275Y/S247N mutant variant of neuraminidase from H1N1 influenza virus with oseltamivir


Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 42.78 KDa
Source organism: unidentified influenza virus
Expression system: Escherichia coli
  • Canonical: C3W6G3 (Residues: 82-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 85.778Å b: 127.538Å c: 96.773Å
α: 90° β: 93.24° γ: 90°
R R work R free
0.229 0.229 0.261
Expression system: Escherichia coli