5nyz

X-ray diffraction
2.5Å resolution

Twist and induce: Dissecting the link between the enzymatic activity and the SaPI inducing capacity of the phage 80 dUTPase. D95E mutant from dUTPase 80alpha phage.

Released:

Function and Biology Details

Reaction catalysed:
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dUTPase Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.13 KDa
Source organism: Staphylococcus virus 80alpha
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4ZF98 (Residues: 1-170; Coverage: 100%)
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P213
Unit cell:
a: 87.27Å b: 87.27Å c: 87.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.281
Expression system: Escherichia coli BL21(DE3)