› 5nyg

X-ray diffraction
2.4Å resolution

Anbu (Gly-1) mutant from Hyphomicrobium sp. strain MC1 - SG P2(1)2(1)2(1)

Released:
DOI: 10.2210/pdb5nyg/pdb
PDB entry 5nyg coloured by chain, front view.
PDB entry 5nyg coloured by chain, side view.
PDB entry 5nyg coloured by chain, top view.
Source organism: Hyphomicrobium sp. MC1
Primary publication:
On the Trails of the Proteasome Fold: Structural and Functional Analysis of the Ancestral β-Subunit Protein Anbu.
Vielberg MT, Bauer VC, Groll M
J Mol Biol 430 628-640 (2018)
PMID: 29355501

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-123707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 245 amino acids
Theoretical weight: 27.54 KDa
Source organism: Hyphomicrobium sp. MC1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F8JB59 (Residues: 2-244; Coverage: 100%)
Gene name: HYPMC_4374
Sequence domains: Proteasome subunit

Ligands and Environments

1 bound ligand:
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 103.25Å b: 116.3Å c: 197.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

The proteasome as a druggable target with multiple therapeutic potentialities: Cutting and non-cutting edges.
Tundo et al. (2020)
1 more

1 mention without citation

The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.
Piasecka et al. (2018)