› 5nxx

X-ray diffraction
2.2Å resolution

Crystal structure of OpuAC from B. subtilis in complex with Arsenobetaine

Released:
DOI: 10.2210/pdb5nxx/pdb
PDB 5nxx coloured by chain and viewed from the front.
PDB 5nxx coloured by chain and viewed from the side.
PDB 5nxx coloured by chain and viewed from the top.
Source organism: Bacillus subtilis
Primary publication:
Arsenobetaine: an ecophysiologically important organoarsenical confers cytoprotection against osmotic stress and growth temperature extremes.
Hoffmann T, Warmbold B, Smits SHJ, Tschapek B, Ronzheimer S, Bashir A, Chen C, Rolbetzki A, Pittelkow M, Jebbar M, Seubert A, Schmitt L, Bremer E
Environ. Microbiol. 20 305-323 (2018)
PMID: 29159878

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine betaine-binding protein OpuAC Chains: C, D
Molecule details ›
Chains: C, D
Length: 268 amino acids
Theoretical weight: 29.78 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46922 (Residues: 26-293; Coverage: 98%)
Gene names: BSU03000, opuAC
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 3Q7 2 x 3Q7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 88.1Å b: 30Å c: 106.1Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.188 0.184 0.282
Expression system: Escherichia coli

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Citations

1 review citation

Interactions with Arsenic: Mechanisms of Toxicity and Cellular Resistance in Eukaryotic Microorganisms.
De Francisco et al. (2021)
13 other articles