› 5nxq

X-ray diffraction
2.41Å resolution

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to a stapled Sld5 CIP

Released:
DOI: 10.2210/pdb5nxq/pdb
PDB entry 5nxq coloured by chain, front view.
PDB entry 5nxq coloured by chain, side view.
PDB entry 5nxq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Targeting the Genome-Stability Hub Ctf4 by Stapled-Peptide Design.
Wu Y, Villa F, Maman J, Lau YH, Dobnikar L, Simon AC, Labib K, Spring DR, Pellegrini L
Angew Chem Int Ed Engl 56 12866-12872 (2017)
PMID: 28815832

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169559 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase alpha-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 479 amino acids
Theoretical weight: 54.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01454 (Residues: 471-927; Coverage: 49%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Sequence domains:
DNA replication complex GINS protein SLD5 Chains: D, E
Molecule details ›
Chains: D, E
Length: 19 amino acids
Theoretical weight: 2.31 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: Q03406 (Residues: 1-19; Coverage: 7%)
Gene names: SLD5, YDR489W

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 88.679Å b: 100.287Å c: 219.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.211
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Using Peptidomimetics and Constrained Peptides as Valuable Tools for Inhibiting Proteinā»Protein Interactions.
Robertson et al. (2018)
1 more

1 mention without citation

Stapled Peptides Inhibitors: A New Window for Target Drug Discovery.
Ali et al. (2019)