› 5nw7

X-ray diffraction
1.85Å resolution

Crystal structure of candida albicans phosphomannose isomerase in complex with inhibitor

Released:
DOI: 10.2210/pdb5nw7/pdb
PDB entry 5nw7 coloured by chain, front view.
PDB entry 5nw7 coloured by chain, side view.
PDB entry 5nw7 coloured by chain, top view.
Source organism: Candida albicans
Primary publication:
Crystal structure of phosphomannose isomerase from Candida albicans complexed with 5-phospho-d-arabinonhydrazide.
Ahmad L, Plancqueel S, Dubosclard V, Lazar N, Ghattas W, Li de la Sierra-Gallay I, van Tilbeurgh H, Salmon L
FEBS Lett 592 1667-1680 (2018)
PMID: 29687459

Function and Biology Details

Reaction catalysed: 
D-mannose 6-phosphate = D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannose-6-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 50.53 KDa
Source organism: Candida albicans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34948 (Residues: 1-441; Coverage: 100%)
Gene names: CAALFM_C209640WA, CaO19.1390, MANA, PMI1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand 9C2 1 x 9C2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 87.97Å b: 107.86Å c: 44.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.207
Expression system: Escherichia coli

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Citations

1 review citation

Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.
Castro et al. (2023)