5nvo

X-ray diffraction
2.4Å resolution

Human DNMT3B PWWP domain in complex with choline

Released:
Source organism: Homo sapiens
Entry authors: Rondelet G, Wouters J

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 3B Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBC3 (Residues: 206-355; Coverage: 18%)
Gene name: DNMT3B
Sequence domains: PWWP domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 74.27Å b: 74.27Å c: 158.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.207 0.244
Expression system: Escherichia coli BL21(DE3)