Structure analysis

Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex

Electron Microscopy
3.8Å resolution
Assembly composition:
hetero octamer (preferred)
Entry contents: 5 distinct polypeptide molecules
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero octamer
Accessible surface area: 180138.04 Å2
Buried surface area: 31477.47 Å2
Dissociation area: 1,186.32 Å2
Dissociation energy (ΔGdiss): 5.6 kcal/mol
Dissociation entropy (TΔSdiss): 15.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-119071

Macromolecules

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Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Pfam: RNA polymerase Rpb6
InterPro:
CATH: Eukaryotic RPB6 RNA polymerase subunit

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Chain: M
Length: 573 amino acids
Theoretical weight: 62.16 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06223 (Residues: 1-117, 118-305, 306-414, 415-477; Coverage: 100%)
Gene names: ntrA, rpoN
Pfam:
InterPro:

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Name: Non-Template promoter DNA
Representative chains: F
Length: 63 nucleotides
Theoretical weight: 19.4 KDa

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Name: Template DNA promoter
Representative chains: G
Length: 63 nucleotides
Theoretical weight: 19.44 KDa

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