› 5nsm

X-ray diffraction
2.4Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosol aminopeptidase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 521 amino acids
Theoretical weight: 55.42 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli
UniProt:
  • Canonical: Q385B0 (Residues: 1-521; Coverage: 100%)
Gene name: Tb11.02.4440
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BCT 12 x BCT
Ligand MN 24 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 87.368Å b: 143.76Å c: 270.085Å
α: 90° β: 95.47° γ: 90°
R-values:
R R work R free
0.207 0.206 0.225
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Uptake and Metabolism of Amino Acids, and Their Unique Role in the Biology of Pathogenic Trypanosomatids.
Marchese et al. (2018)
3 other articles

1 mention without citation

Structural Characterization of Acidic M17 Leucine Aminopeptidases from the TriTryps and Evaluation of Their Role in Nutrient Starvation in Trypanosoma brucei.
Timm et al. (2017)