› 5nrs

X-ray diffraction
2.3Å resolution

Human DNMT3B PWWP domain in complex with N,N-bis(2-hydroxypropyl)ethanolamine

Released:
DOI: 10.2210/pdb5nrs/pdb
PDB 5nrs coloured by chain and viewed from the front.
PDB 5nrs coloured by chain and viewed from the side.
PDB 5nrs coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Rondelet G, Wouters J

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 3B Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBC3 (Residues: 206-355; Coverage: 18%)
Gene name: DNMT3B
Sequence domains: PWWP domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand 962 2 x 962
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 74.93Å b: 74.93Å c: 157.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.195 0.248
Expression system: Escherichia coli BL21(DE3)

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