› 5nqd

X-ray diffraction
2.2Å resolution

Arsenite oxidase AioAB from Rhizobium sp. str. NT-26 mutant AioBF108A

Released:
DOI: 10.2210/pdb5nqd/pdb
PDB entry 5nqd coloured by chain, front view.
PDB entry 5nqd coloured by chain, side view.
PDB entry 5nqd coloured by chain, top view.
Source organism: Pseudorhizobium banfieldiae
Entry authors: Santos-Silva T, Romao M, Vieira M, Marques AT

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125759 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AroA Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 843 amino acids
Theoretical weight: 93.18 KDa
Source organism: Pseudorhizobium banfieldiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6VAL8 (Residues: 2-844; Coverage: 100%)
Gene names: NT26_p10030, aioA, aroA
Sequence domains:
Structure domains:
Rieske domain-containing protein Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 132 amino acids
Theoretical weight: 14.02 KDa
Source organism: Pseudorhizobium banfieldiae
Expression system: Escherichia coli
UniProt:
  • Canonical: L0NMC5 (Residues: 44-175; Coverage: 75%)
Gene names: NT26_p10029, aioB
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments


Cofactor: Ligand MGD 8 x MGD
10 bound ligands:
Ligand O 4 x O
Ligand 4MO 4 x 4MO
Ligand F3S 4 x F3S
Ligand SO4 12 x SO4
Ligand PGE 1 x PGE
Ligand GOL 5 x GOL
Ligand FES 4 x FES
Ligand EDO 3 x EDO
Ligand PEG 1 x PEG
Ligand P33 2 x P33
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Unit cell:
a: 141.408Å b: 148.665Å c: 232.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.217
Expression system: Escherichia coli

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