5npk

X-ray diffraction
1.98Å resolution

1.98A STRUCTURE OF THIOPHENE1 WITH S.AUREUS DNA GYRASE AND DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA gyrase subunit A; DNA gyrase subunit B Chains: B, D, b, d
Molecule details ›
Chains: B, D, b, d
Length: 692 amino acids
Theoretical weight: 78.11 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99XG5 (Residues: 2-491; Coverage: 55%)
  • Canonical: P66937 (Residues: 409-543, 580-642; Coverage: 31%)
Gene names: SA0005, SA0006, gyrA, gyrB
Sequence domains:
DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') Chains: E, F, e, f
Molecule details ›
Chains: E, F, e, f
Length: 20 nucleotides
Theoretical weight: 6.13 KDa

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 89.442Å b: 120.68Å c: 168.899Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.155 0.154 0.183
Expression system: Escherichia coli