› 5nnw

X-ray diffraction
1.54Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
25 kDa protein elicitor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 219 amino acids
Theoretical weight: 24.13 KDa
Source organism: Pythium aphanidermatum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SPD4 (Residues: 22-234; Coverage: 98%)
Gene name: SD21-1
Sequence domains: Necrosis inducing protein (NPP1)

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GCS 1 x GCS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2221
Unit cell:
a: 114.996Å b: 122.285Å c: 121.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions.
Li et al. (2020)
6 more

1 mention without citation

Molecular basis for functional diversity among microbial Nep1-like proteins.
Lenarčič et al. (2019)