› 5nna

X-ray diffraction
1.5Å resolution

Isatin hydrolase A (IHA) from Labrenzia aggregata bound to benzyl benzoate

Released:
DOI: 10.2210/pdb5nna/pdb
PDB entry 5nna coloured by chain, front view.
PDB entry 5nna coloured by chain, side view.
PDB entry 5nna coloured by chain, top view.
Source organism: Roseibium aggregatum
Primary publication:
A fundamental catalytic difference between zinc and manganese dependent enzymes revealed in a bacterial isatin hydrolase.
Sommer T, Bjerregaard-Andersen K, Uribe L, Etzerodt M, Diezemann G, Gauss J, Cascella M, Morth JP
Sci Rep 8 13104 (2018)
PMID: 30166577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cyclase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 28.81 KDa
Source organism: Roseibium aggregatum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0P0F0 (Residues: 2-257; Coverage: 100%)
Gene name: SIAM614_30646
Sequence domains: Putative cyclase
Structure domains: Putative cyclase

Ligands and Environments

5 bound ligands:
Ligand BZM 4 x BZM
Ligand MN 4 x MN
Ligand BTB 1 x BTB
Ligand 1PE 1 x 1PE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 37.69Å b: 70.67Å c: 92.92Å
α: 108.23° β: 95.94° γ: 101.48°
R-values:
R R work R free
0.149 0.148 0.177
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Systematic Structure-Based Search for Ochratoxin-Degrading Enzymes in Proteomes from Filamentous Fungi.
Leitão et al. (2021)
3 more

2 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
1 more